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KMID : 0984920170190020056
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Journal of Skin Barrier Research
2017 Volume.19 No. 2 p.56 ~ p.56
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TRPV3 as Potential Therapeutic Target for Post-burn Pruritus
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Choi Yong-Won
Jung Min-Je
Son Jee-Hee
Cho Yong-Se
Chung Bo-Young
Park Chun-Wook
Kim Hye-One
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Abstract
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Post-burn pruritus is a common distressing sequela of burn scars. Empirical antipruritic treatments usually fail to have a satisfactory outcome because of their limited selectivity and possible side effects. Therefore, novel drug target need to be identified. We aimed to investigate the possible role of protease-activated receptor 2 (PAR2) and transient receptor potential vanniloid 3 (TRPV3), along with relations of TRPV3 to thymic stromal lymphopoietin (TSLP). Specimens from normal or burn scarred with or without pruritus tissue, were obtained from burn patients who underwent plastic surgery. In each sample, the keratinocytes were isolated and cultured, and the intracellular calcium concentration at the time of stimulation of each factor was quantified and the interaction was screened. PAR2 function was reduced with antagonism of TRPV3. And PAR2 downstream pathway protein kinase A (PKA) and protein kinase C (PKC) were necessary for TRPV3 to function. TSLP mRNA and protein, and TSLPR protein expressions were increased in scars with postburn pruritus compared to scars without it or normal tissues. In addition, TRPV3 activation induced increased TSLP expression. Conclusively, TRPV3 may contribute to pruritus in burn scar through TSLP, and can be considered for potential therapeutic target for post-burn pruritus.
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